Dr.Pooja Khamar, K17849
Purpose: To study the effect of SAHA (suberanilohydroxamic acid) treatment on wound healing response and haze post refractive surgery.
Methods: In the first arm of a dual arm study, intra-op SMILE lenticules were treated with SAHA and TGF-beta. In the second arm, refractive surgery was performed, in donor human eyes unsuitable for transplantation, to induce wound healing response. These tissues were then treated with SAHA and compared to controls. In both arms, gene expression analysis of fibrotic, extracellular matrix and inflammation genes was performed by QPCR.
Results: SAHA significantly reduced levels of CTGF, TGF-beta and alpha-SMA. However, levels of IL6 and Lysyl Oxidase were not affected. Donor tissues treated with SAHA and MMC post-surgery showed reduction in fibrotic gene expression in SAHA samples in short duration times.
Conclusion: SAHA treatment may therefore be a novel treatment modality to prevent haze post refractive surgery with less collateral damage then MMC.
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