Dr.Sinu Thulaseedharan Sinumols, S07971
AIM: To analyze the histomorphological effects of trypan blue on Lens Epithelial cells (LECs) and the Basement Membrane on direct exposure.METHODS: Analytical cross sectional case control study. Anterior capsule specimens of 14 Patients were studied. Two specimens of anterior capsule from the same eye form the case and control. Control specimen (sample A) was removed first, after the routine external staining with trypan blue 0.06 %( w/v) for 10 seconds. Then trypan blue was injected under the remaining anterior capsule and case specimen (sample B) was obtained after direct contact of trypan blue to the internal surface (lens epithelial cells) for 1 minute. Histomorphological (qualitative) examination and quantitative analysis of both specimens were done.RESULTS: Qualitative data analysis showed partial and complete detachment of LECs, degeneration of LECs and nuclear smudging were significantly higher in Sample B. Quantitative analysis showed decrease in cell density in sample B.
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